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Merck & Co f95 pan-deimination antibody
Deiminated proteins identified by <t> F95 </t> enrichment and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis in coelomic fluid (CF; pool of 3 individual samples) and EVs (pool of 3 individual samples) of purple sea urchin ( Strongylocentrotus purpuratus ); hits are run against the UniProt Echinoidea database. Proteins identified as <t> deimination </t> hits in EVs, coelomic fluid (CF), or both are indicated by a tick (v) for the columns representing EVs and CF, respectively. Uncharacterised hits with an annotated secondary hit are included and indicated in brackets; other unidentified protein hits are not included in this table. Further detailed analysis on number of matches and total score is provided in <xref ref-type= Supplementary Tables S2 and S3 , respectively, as well as full LC-MS/MS results (also including data from a common contaminant database; cRAP 20,190,401; 125 sequences; 41,129 residues) in Supplementary Tables S4 and S5 , respectively." width="250" height="auto" />
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1) Product Images from "Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology"

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology

Journal: Biology

doi: 10.3390/biology10090866

Deiminated proteins identified by  F95  enrichment and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis in coelomic fluid (CF; pool of 3 individual samples) and EVs (pool of 3 individual samples) of purple sea urchin ( Strongylocentrotus purpuratus ); hits are run against the UniProt Echinoidea database. Proteins identified as  deimination  hits in EVs, coelomic fluid (CF), or both are indicated by a tick (v) for the columns representing EVs and CF, respectively. Uncharacterised hits with an annotated secondary hit are included and indicated in brackets; other unidentified protein hits are not included in this table. Further detailed analysis on number of matches and total score is provided in <xref ref-type= Supplementary Tables S2 and S3 , respectively, as well as full LC-MS/MS results (also including data from a common contaminant database; cRAP 20,190,401; 125 sequences; 41,129 residues) in Supplementary Tables S4 and S5 , respectively." title="Deiminated proteins identified by F95 enrichment and liquid chromatography with tandem ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: Deiminated proteins identified by F95 enrichment and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis in coelomic fluid (CF; pool of 3 individual samples) and EVs (pool of 3 individual samples) of purple sea urchin ( Strongylocentrotus purpuratus ); hits are run against the UniProt Echinoidea database. Proteins identified as deimination hits in EVs, coelomic fluid (CF), or both are indicated by a tick (v) for the columns representing EVs and CF, respectively. Uncharacterised hits with an annotated secondary hit are included and indicated in brackets; other unidentified protein hits are not included in this table. Further detailed analysis on number of matches and total score is provided in Supplementary Tables S2 and S3 , respectively, as well as full LC-MS/MS results (also including data from a common contaminant database; cRAP 20,190,401; 125 sequences; 41,129 residues) in Supplementary Tables S4 and S5 , respectively.

Techniques Used: Liquid Chromatography, Mass Spectrometry

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Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions
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Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
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Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei )
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Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: F95 pan-deimination 182 antibody (MABN328, Merck, 1/1000); anti-PAD2, (ab50257, Abcam, 1/1000); CD63 (ab216130, 183 1/1000); Flot-1 (ab41927, 1/2000).

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Release® v2.0 immunoprecipitation kit (Merck, U.K.) was used together with the F95 191 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-192 citrullinated peptide and specifically detects proteins modified by citrullination [51], to isolate total 193 deiminated proteins from seal sera and serum-derived EVs.

Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 (ab50257, Abcam, diluted 1/1000 in TBS-T), PAD3 (ab50246, diluted 1/1000 in TBS-T) and PAD4 (ab50332, diluted 1/1000 in TBS-T) antibodies, for detection of putative PAD protein homologues.

Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the F95 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-citrullinated peptide and specifically detects proteins modified by citrullination [ ].

Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus).
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Mass Spectrometry:

Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions
Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the F95 pan-deimination antibody (MABN328, Merck) and the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK).

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the F95 pan-deimination antibody (MABN328, Merck, Feltham UK), which detects citrullinated proteins and has been developed against a deca-citrullinated peptide [ ], in conjunction with the Catch and Release ® v2.0 immunoprecipitation kit (Merck), according to previously described methods [ ].

Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 antibody (anti-PAD2, ab50257, Abcam, Cambridge, UK; diluted 1/1000), for detection of putative PAD protein homologues, due to PAD2 being the most conserved PAD isozyme and the anti-human PAD2 antibody was previously shown to cross-react with PADs across taxa [ , , , , , , , , , , , , , ].

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: F95 pan-deimination 182 antibody (MABN328, Merck, 1/1000); anti-PAD2, (ab50257, Abcam, 1/1000); CD63 (ab216130, 183 1/1000); Flot-1 (ab41927, 1/2000).

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Release® v2.0 immunoprecipitation kit (Merck, U.K.) was used together with the F95 191 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-192 citrullinated peptide and specifically detects proteins modified by citrullination [51], to isolate total 193 deiminated proteins from seal sera and serum-derived EVs.

Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 (ab50257, Abcam, diluted 1/1000 in TBS-T), PAD3 (ab50246, diluted 1/1000 in TBS-T) and PAD4 (ab50332, diluted 1/1000 in TBS-T) antibodies, for detection of putative PAD protein homologues.

Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the F95 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-citrullinated peptide and specifically detects proteins modified by citrullination [ ].

Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus).
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Sequencing:

Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions
Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the F95 pan-deimination antibody (MABN328, Merck) and the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK).

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the F95 pan-deimination antibody (MABN328, Merck, Feltham UK), which detects citrullinated proteins and has been developed against a deca-citrullinated peptide [ ], in conjunction with the Catch and Release ® v2.0 immunoprecipitation kit (Merck), according to previously described methods [ ].

Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 antibody (anti-PAD2, ab50257, Abcam, Cambridge, UK; diluted 1/1000), for detection of putative PAD protein homologues, due to PAD2 being the most conserved PAD isozyme and the anti-human PAD2 antibody was previously shown to cross-react with PADs across taxa [ , , , , , , , , , , , , , ].

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: F95 pan-deimination 182 antibody (MABN328, Merck, 1/1000); anti-PAD2, (ab50257, Abcam, 1/1000); CD63 (ab216130, 183 1/1000); Flot-1 (ab41927, 1/2000).

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Release® v2.0 immunoprecipitation kit (Merck, U.K.) was used together with the F95 191 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-192 citrullinated peptide and specifically detects proteins modified by citrullination [51], to isolate total 193 deiminated proteins from seal sera and serum-derived EVs.

Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 (ab50257, Abcam, diluted 1/1000 in TBS-T), PAD3 (ab50246, diluted 1/1000 in TBS-T) and PAD4 (ab50332, diluted 1/1000 in TBS-T) antibodies, for detection of putative PAD protein homologues.

Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the F95 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-citrullinated peptide and specifically detects proteins modified by citrullination [ ].

Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus).
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Modification:

Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions
Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the F95 pan-deimination antibody (MABN328, Merck) and the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK).

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the F95 pan-deimination antibody (MABN328, Merck, Feltham UK), which detects citrullinated proteins and has been developed against a deca-citrullinated peptide [ ], in conjunction with the Catch and Release ® v2.0 immunoprecipitation kit (Merck), according to previously described methods [ ].

Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 antibody (anti-PAD2, ab50257, Abcam, Cambridge, UK; diluted 1/1000), for detection of putative PAD protein homologues, due to PAD2 being the most conserved PAD isozyme and the anti-human PAD2 antibody was previously shown to cross-react with PADs across taxa [ , , , , , , , , , , , , , ].

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: F95 pan-deimination 182 antibody (MABN328, Merck, 1/1000); anti-PAD2, (ab50257, Abcam, 1/1000); CD63 (ab216130, 183 1/1000); Flot-1 (ab41927, 1/2000).

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Release® v2.0 immunoprecipitation kit (Merck, U.K.) was used together with the F95 191 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-192 citrullinated peptide and specifically detects proteins modified by citrullination [51], to isolate total 193 deiminated proteins from seal sera and serum-derived EVs.

Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 (ab50257, Abcam, diluted 1/1000 in TBS-T), PAD3 (ab50246, diluted 1/1000 in TBS-T) and PAD4 (ab50332, diluted 1/1000 in TBS-T) antibodies, for detection of putative PAD protein homologues.

Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the F95 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-citrullinated peptide and specifically detects proteins modified by citrullination [ ].

Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus).
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Incubation:

Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions
Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the F95 pan-deimination antibody (MABN328, Merck) and the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK).

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the F95 pan-deimination antibody (MABN328, Merck, Feltham UK), which detects citrullinated proteins and has been developed against a deca-citrullinated peptide [ ], in conjunction with the Catch and Release ® v2.0 immunoprecipitation kit (Merck), according to previously described methods [ ].

Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 antibody (anti-PAD2, ab50257, Abcam, Cambridge, UK; diluted 1/1000), for detection of putative PAD protein homologues, due to PAD2 being the most conserved PAD isozyme and the anti-human PAD2 antibody was previously shown to cross-react with PADs across taxa [ , , , , , , , , , , , , , ].

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: F95 pan-deimination 182 antibody (MABN328, Merck, 1/1000); anti-PAD2, (ab50257, Abcam, 1/1000); CD63 (ab216130, 183 1/1000); Flot-1 (ab41927, 1/2000).

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Release® v2.0 immunoprecipitation kit (Merck, U.K.) was used together with the F95 191 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-192 citrullinated peptide and specifically detects proteins modified by citrullination [51], to isolate total 193 deiminated proteins from seal sera and serum-derived EVs.

Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 (ab50257, Abcam, diluted 1/1000 in TBS-T), PAD3 (ab50246, diluted 1/1000 in TBS-T) and PAD4 (ab50332, diluted 1/1000 in TBS-T) antibodies, for detection of putative PAD protein homologues.

Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the F95 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-citrullinated peptide and specifically detects proteins modified by citrullination [ ].

Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus).
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Binding Assay:

Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions
Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the F95 pan-deimination antibody (MABN328, Merck) and the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK).

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the F95 pan-deimination antibody (MABN328, Merck, Feltham UK), which detects citrullinated proteins and has been developed against a deca-citrullinated peptide [ ], in conjunction with the Catch and Release ® v2.0 immunoprecipitation kit (Merck), according to previously described methods [ ].

Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 antibody (anti-PAD2, ab50257, Abcam, Cambridge, UK; diluted 1/1000), for detection of putative PAD protein homologues, due to PAD2 being the most conserved PAD isozyme and the anti-human PAD2 antibody was previously shown to cross-react with PADs across taxa [ , , , , , , , , , , , , , ].

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: F95 pan-deimination 182 antibody (MABN328, Merck, 1/1000); anti-PAD2, (ab50257, Abcam, 1/1000); CD63 (ab216130, 183 1/1000); Flot-1 (ab41927, 1/2000).

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Release® v2.0 immunoprecipitation kit (Merck, U.K.) was used together with the F95 191 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-192 citrullinated peptide and specifically detects proteins modified by citrullination [51], to isolate total 193 deiminated proteins from seal sera and serum-derived EVs.

Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 (ab50257, Abcam, diluted 1/1000 in TBS-T), PAD3 (ab50246, diluted 1/1000 in TBS-T) and PAD4 (ab50332, diluted 1/1000 in TBS-T) antibodies, for detection of putative PAD protein homologues.

Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the F95 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-citrullinated peptide and specifically detects proteins modified by citrullination [ ].

Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus).
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Silver Staining:

Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions
Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the F95 pan-deimination antibody (MABN328, Merck) and the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK).

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the F95 pan-deimination antibody (MABN328, Merck, Feltham UK), which detects citrullinated proteins and has been developed against a deca-citrullinated peptide [ ], in conjunction with the Catch and Release ® v2.0 immunoprecipitation kit (Merck), according to previously described methods [ ].

Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 antibody (anti-PAD2, ab50257, Abcam, Cambridge, UK; diluted 1/1000), for detection of putative PAD protein homologues, due to PAD2 being the most conserved PAD isozyme and the anti-human PAD2 antibody was previously shown to cross-react with PADs across taxa [ , , , , , , , , , , , , , ].

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: F95 pan-deimination 182 antibody (MABN328, Merck, 1/1000); anti-PAD2, (ab50257, Abcam, 1/1000); CD63 (ab216130, 183 1/1000); Flot-1 (ab41927, 1/2000).

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Release® v2.0 immunoprecipitation kit (Merck, U.K.) was used together with the F95 191 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-192 citrullinated peptide and specifically detects proteins modified by citrullination [51], to isolate total 193 deiminated proteins from seal sera and serum-derived EVs.

Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 (ab50257, Abcam, diluted 1/1000 in TBS-T), PAD3 (ab50246, diluted 1/1000 in TBS-T) and PAD4 (ab50332, diluted 1/1000 in TBS-T) antibodies, for detection of putative PAD protein homologues.

Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the F95 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-citrullinated peptide and specifically detects proteins modified by citrullination [ ].

Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus).
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Immunodetection:

Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions
Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the F95 pan-deimination antibody (MABN328, Merck) and the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK).

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the F95 pan-deimination antibody (MABN328, Merck, Feltham UK), which detects citrullinated proteins and has been developed against a deca-citrullinated peptide [ ], in conjunction with the Catch and Release ® v2.0 immunoprecipitation kit (Merck), according to previously described methods [ ].

Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 antibody (anti-PAD2, ab50257, Abcam, Cambridge, UK; diluted 1/1000), for detection of putative PAD protein homologues, due to PAD2 being the most conserved PAD isozyme and the anti-human PAD2 antibody was previously shown to cross-react with PADs across taxa [ , , , , , , , , , , , , , ].

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: F95 pan-deimination 182 antibody (MABN328, Merck, 1/1000); anti-PAD2, (ab50257, Abcam, 1/1000); CD63 (ab216130, 183 1/1000); Flot-1 (ab41927, 1/2000).

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Release® v2.0 immunoprecipitation kit (Merck, U.K.) was used together with the F95 191 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-192 citrullinated peptide and specifically detects proteins modified by citrullination [51], to isolate total 193 deiminated proteins from seal sera and serum-derived EVs.

Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 (ab50257, Abcam, diluted 1/1000 in TBS-T), PAD3 (ab50246, diluted 1/1000 in TBS-T) and PAD4 (ab50332, diluted 1/1000 in TBS-T) antibodies, for detection of putative PAD protein homologues.

Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the F95 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-citrullinated peptide and specifically detects proteins modified by citrullination [ ].

Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus).
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Western Blot:

Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions
Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the F95 pan-deimination antibody (MABN328, Merck) and the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK).

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the F95 pan-deimination antibody (MABN328, Merck, Feltham UK), which detects citrullinated proteins and has been developed against a deca-citrullinated peptide [ ], in conjunction with the Catch and Release ® v2.0 immunoprecipitation kit (Merck), according to previously described methods [ ].

Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 antibody (anti-PAD2, ab50257, Abcam, Cambridge, UK; diluted 1/1000), for detection of putative PAD protein homologues, due to PAD2 being the most conserved PAD isozyme and the anti-human PAD2 antibody was previously shown to cross-react with PADs across taxa [ , , , , , , , , , , , , , ].

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: F95 pan-deimination 182 antibody (MABN328, Merck, 1/1000); anti-PAD2, (ab50257, Abcam, 1/1000); CD63 (ab216130, 183 1/1000); Flot-1 (ab41927, 1/2000).

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Release® v2.0 immunoprecipitation kit (Merck, U.K.) was used together with the F95 191 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-192 citrullinated peptide and specifically detects proteins modified by citrullination [51], to isolate total 193 deiminated proteins from seal sera and serum-derived EVs.

Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 (ab50257, Abcam, diluted 1/1000 in TBS-T), PAD3 (ab50246, diluted 1/1000 in TBS-T) and PAD4 (ab50332, diluted 1/1000 in TBS-T) antibodies, for detection of putative PAD protein homologues.

Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the F95 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-citrullinated peptide and specifically detects proteins modified by citrullination [ ].

Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus).
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Protease Inhibitor:

Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions
Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the F95 pan-deimination antibody (MABN328, Merck) and the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK).

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the F95 pan-deimination antibody (MABN328, Merck, Feltham UK), which detects citrullinated proteins and has been developed against a deca-citrullinated peptide [ ], in conjunction with the Catch and Release ® v2.0 immunoprecipitation kit (Merck), according to previously described methods [ ].

Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 antibody (anti-PAD2, ab50257, Abcam, Cambridge, UK; diluted 1/1000), for detection of putative PAD protein homologues, due to PAD2 being the most conserved PAD isozyme and the anti-human PAD2 antibody was previously shown to cross-react with PADs across taxa [ , , , , , , , , , , , , , ].

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: F95 pan-deimination 182 antibody (MABN328, Merck, 1/1000); anti-PAD2, (ab50257, Abcam, 1/1000); CD63 (ab216130, 183 1/1000); Flot-1 (ab41927, 1/2000).

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Release® v2.0 immunoprecipitation kit (Merck, U.K.) was used together with the F95 191 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-192 citrullinated peptide and specifically detects proteins modified by citrullination [51], to isolate total 193 deiminated proteins from seal sera and serum-derived EVs.

Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 (ab50257, Abcam, diluted 1/1000 in TBS-T), PAD3 (ab50246, diluted 1/1000 in TBS-T) and PAD4 (ab50332, diluted 1/1000 in TBS-T) antibodies, for detection of putative PAD protein homologues.

Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the F95 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-citrullinated peptide and specifically detects proteins modified by citrullination [ ].

Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus).
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Coagulation:

Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions
Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the F95 pan-deimination antibody (MABN328, Merck) and the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK).

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the F95 pan-deimination antibody (MABN328, Merck, Feltham UK), which detects citrullinated proteins and has been developed against a deca-citrullinated peptide [ ], in conjunction with the Catch and Release ® v2.0 immunoprecipitation kit (Merck), according to previously described methods [ ].

Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 antibody (anti-PAD2, ab50257, Abcam, Cambridge, UK; diluted 1/1000), for detection of putative PAD protein homologues, due to PAD2 being the most conserved PAD isozyme and the anti-human PAD2 antibody was previously shown to cross-react with PADs across taxa [ , , , , , , , , , , , , , ].

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: F95 pan-deimination 182 antibody (MABN328, Merck, 1/1000); anti-PAD2, (ab50257, Abcam, 1/1000); CD63 (ab216130, 183 1/1000); Flot-1 (ab41927, 1/2000).

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Release® v2.0 immunoprecipitation kit (Merck, U.K.) was used together with the F95 191 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-192 citrullinated peptide and specifically detects proteins modified by citrullination [51], to isolate total 193 deiminated proteins from seal sera and serum-derived EVs.

Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 (ab50257, Abcam, diluted 1/1000 in TBS-T), PAD3 (ab50246, diluted 1/1000 in TBS-T) and PAD4 (ab50332, diluted 1/1000 in TBS-T) antibodies, for detection of putative PAD protein homologues.

Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the F95 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-citrullinated peptide and specifically detects proteins modified by citrullination [ ].

Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus).
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Blocking Assay:

Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions
Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the F95 pan-deimination antibody (MABN328, Merck) and the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK).

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the F95 pan-deimination antibody (MABN328, Merck, Feltham UK), which detects citrullinated proteins and has been developed against a deca-citrullinated peptide [ ], in conjunction with the Catch and Release ® v2.0 immunoprecipitation kit (Merck), according to previously described methods [ ].

Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 antibody (anti-PAD2, ab50257, Abcam, Cambridge, UK; diluted 1/1000), for detection of putative PAD protein homologues, due to PAD2 being the most conserved PAD isozyme and the anti-human PAD2 antibody was previously shown to cross-react with PADs across taxa [ , , , , , , , , , , , , , ].

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: F95 pan-deimination 182 antibody (MABN328, Merck, 1/1000); anti-PAD2, (ab50257, Abcam, 1/1000); CD63 (ab216130, 183 1/1000); Flot-1 (ab41927, 1/2000).

Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).
Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Release® v2.0 immunoprecipitation kit (Merck, U.K.) was used together with the F95 191 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-192 citrullinated peptide and specifically detects proteins modified by citrullination [51], to isolate total 193 deiminated proteins from seal sera and serum-derived EVs.

Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus )
Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: F95 pan-deimination antibody (MABN328, Merck; diluted 1/1000 in TBS-T) and anti-human PAD2 (ab50257, Abcam, diluted 1/1000 in TBS-T), PAD3 (ab50246, diluted 1/1000 in TBS-T) and PAD4 (ab50332, diluted 1/1000 in TBS-T) antibodies, for detection of putative PAD protein homologues.

Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the F95 pan-deimination antibody (MABN328, Merck), which has been developed against a deca-citrullinated peptide and specifically detects proteins modified by citrullination [ ].

Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus).
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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Deiminated proteins identified by <t> F95 </t> enrichment and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis in coelomic fluid (CF; pool of 3 individual samples) and EVs (pool of 3 individual samples) of purple sea urchin ( Strongylocentrotus purpuratus ); hits are run against the UniProt Echinoidea database. Proteins identified as <t> deimination </t> hits in EVs, coelomic fluid (CF), or both are indicated by a tick (v) for the columns representing EVs and CF, respectively. Uncharacterised hits with an annotated secondary hit are included and indicated in brackets; other unidentified protein hits are not included in this table. Further detailed analysis on number of matches and total score is provided in <xref ref-type= Supplementary Tables S2 and S3 , respectively, as well as full LC-MS/MS results (also including data from a common contaminant database; cRAP 20,190,401; 125 sequences; 41,129 residues) in Supplementary Tables S4 and S5 , respectively." width="250" height="auto" />
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Image Search Results


Deiminated proteins identified by  F95  enrichment and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis in coelomic fluid (CF; pool of 3 individual samples) and EVs (pool of 3 individual samples) of purple sea urchin ( Strongylocentrotus purpuratus ); hits are run against the UniProt Echinoidea database. Proteins identified as  deimination  hits in EVs, coelomic fluid (CF), or both are indicated by a tick (v) for the columns representing EVs and CF, respectively. Uncharacterised hits with an annotated secondary hit are included and indicated in brackets; other unidentified protein hits are not included in this table. Further detailed analysis on number of matches and total score is provided in <xref ref-type= Supplementary Tables S2 and S3 , respectively, as well as full LC-MS/MS results (also including data from a common contaminant database; cRAP 20,190,401; 125 sequences; 41,129 residues) in Supplementary Tables S4 and S5 , respectively." width="100%" height="100%">

Journal: Biology

Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology

doi: 10.3390/biology10090866

Figure Lengend Snippet: Deiminated proteins identified by F95 enrichment and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis in coelomic fluid (CF; pool of 3 individual samples) and EVs (pool of 3 individual samples) of purple sea urchin ( Strongylocentrotus purpuratus ); hits are run against the UniProt Echinoidea database. Proteins identified as deimination hits in EVs, coelomic fluid (CF), or both are indicated by a tick (v) for the columns representing EVs and CF, respectively. Uncharacterised hits with an annotated secondary hit are included and indicated in brackets; other unidentified protein hits are not included in this table. Further detailed analysis on number of matches and total score is provided in Supplementary Tables S2 and S3 , respectively, as well as full LC-MS/MS results (also including data from a common contaminant database; cRAP 20,190,401; 125 sequences; 41,129 residues) in Supplementary Tables S4 and S5 , respectively.

Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the F95 pan-deimination antibody (MABN328, Merck, Feltham UK), which detects citrullinated proteins and has been developed against a deca-citrullinated peptide [ ], in conjunction with the Catch and Release ® v2.0 immunoprecipitation kit (Merck), according to previously described methods [ ].

Techniques: Liquid Chromatography, Mass Spectrometry

Deiminated proteins in serum extracellular vesicles (EVs) of halibut ( Hippoglossus hippoglossus L), as identified by  F95-enrichment  in conjunction with LC-MS/MS analysis. Deiminated proteins were isolated from serum-EVs from a pool of n = 4 fish, using immunoprecipitation with the  pan-deimination F95 antibody.  The resulting F95-enriched eluate was then analysed by LC-MS/MS and peak list files submitted to Mascot, using the Teleost UniProt database. Peptide sequence hits are listed, showing the number of sequences for protein hits and total score. Species hit names are indicated. In the case of uncharacterised protein ID, proteins matching the same set of peptides are indicated in brackets. Protein hits highlighted in pink (*) are specific to the F95 enriched EV fraction only. Protein names are written in bold. A full list of protein sequence hits and peptides is further provided in <xref ref-type= Supplementary Table S1 ." width="100%" height="100%">

Journal: International Journal of Molecular Sciences

Article Title: The Proteome and Citrullinome of Hippoglossus hippoglossus Extracellular Vesicles—Novel Insights into Roles of the Serum Secretome in Immune, Gene Regulatory and Metabolic Pathways

doi: 10.3390/ijms22020875

Figure Lengend Snippet: Deiminated proteins in serum extracellular vesicles (EVs) of halibut ( Hippoglossus hippoglossus L), as identified by F95-enrichment in conjunction with LC-MS/MS analysis. Deiminated proteins were isolated from serum-EVs from a pool of n = 4 fish, using immunoprecipitation with the pan-deimination F95 antibody. The resulting F95-enriched eluate was then analysed by LC-MS/MS and peak list files submitted to Mascot, using the Teleost UniProt database. Peptide sequence hits are listed, showing the number of sequences for protein hits and total score. Species hit names are indicated. In the case of uncharacterised protein ID, proteins matching the same set of peptides are indicated in brackets. Protein hits highlighted in pink (*) are specific to the F95 enriched EV fraction only. Protein names are written in bold. A full list of protein sequence hits and peptides is further provided in Supplementary Table S1 .

Article Snippet: The isolation of deiminated/citrullinated proteins from serum-EVs was carried out by immunoprecipitation, using the Catch and Release ® v2.0 Reversible Immunoprecipitation System (Merck, Feltham, UK) according to the manufacturer’s instructions in conjunction with the pan-deimination F95 antibody (MABN328, Merck), which specifically detects proteins modified by citrullination [ ].

Techniques: Isolation, Immunoprecipitation, Sequencing, Northern Blot, Fluorescence In Situ Hybridization