f95 pan-deimination antibody (Merck & Co)
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Supplementary Tables S2 and S3 , respectively, as well as full LC-MS/MS results (also including data from a common contaminant database; cRAP 20,190,401; 125 sequences; 41,129 residues) in F95 Pan Deimination Antibody, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology
Journal: Biology
doi: 10.3390/biology10090866
Supplementary Tables S2 and S3 , respectively, as well as full LC-MS/MS results (also including data from a common contaminant database; cRAP 20,190,401; 125 sequences; 41,129 residues) in Figure Legend Snippet: Deiminated proteins identified by F95 enrichment and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis in coelomic fluid (CF; pool of 3 individual samples) and EVs (pool of 3 individual samples) of purple sea urchin ( Strongylocentrotus purpuratus ); hits are run against the UniProt Echinoidea database. Proteins identified as deimination hits in EVs, coelomic fluid (CF), or both are indicated by a tick (v) for the columns representing EVs and CF, respectively. Uncharacterised hits with an annotated secondary hit are included and indicated in brackets; other unidentified protein hits are not included in this table. Further detailed analysis on number of matches and total score is provided in
Techniques Used: Liquid Chromatography, Mass Spectrometry
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Western Blot:Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei ) Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina). Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina). Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus ) Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus). Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Protease Inhibitor:Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei ) Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina). Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina). Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus ) Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus). Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Coagulation:Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei ) Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina). Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina). Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus ) Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus). Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Blocking Assay:Article Title: Peptidylarginine Deiminase (PAD) and Post-Translational Protein Deimination—Novel Insights into Alveolata Metabolism, Epigenetic Regulation and Host–Pathogen Interactions Article Snippet: Total deiminated proteins were isolated from the protein extracts of the two Alveolata species, using the Article Title: Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin ( Strongylocentrotus purpuratus ) Coelomic Fluid—Novel Insights into Echinodermata Biology Article Snippet: Isolation of total deiminated proteins from sea urchin coelomic fluid and EVs was carried out using the Article Title: Extracellular Vesicles and Post-Translational Protein Deimination Signatures in Mollusca—The Blue Mussel ( Mytilus edulis ), Soft Shell Clam ( Mya arenaria ), Eastern Oyster ( Crassostrea virginica ) and Atlantic Jacknife Clam ( Ensis leei ) Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for Mollusca sera: Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina). Article Snippet: Primary antibodies were 181 diluted in TBS-T and incubation was performed at 4 °C overnight as follows: Article Title: Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina). Article Snippet: 188 189 2.5 Immunoprecipitation and Protein Identification 190 The Catch and Article Title: Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer ( Rangifer tarandus ) Article Snippet: Primary antibody incubation was carried out overnight at 4 °C on a shaking platform using the following antibodies for reindeer plasma: Article Title: Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination Article Snippet: Immunoprecipitation and isolation of deiminated proteins in serum and serum-derived EVs was carried out using the Catch and Release ® v2.0 immunoprecipitation kit (Merck, UK) in conjunction with the Article Title: Extracellular vesicles and post-translational protein deimination signatures in haemolymph of the American lobster (Homarus americanus). Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. |
Supplementary Table S1 ." width="250" height="auto" />